Description

The Real-Time Quantitative PCR System YG1285 // YG1286 is a high-performing qPCR instrument, distinguished by its advanced optical signal collection and robust spectrum separation technology. This system offers precise temperature control, ensuring high-sensitivity detection and exceptional temperature uniformity. Available in 4-channel and 5-channel configurations, it supports a wide range of dye-based and probe-based chemistries. Ideal for absolute quantification, gene expression analysis, genotyping, and high-resolution melting curve analysis, it’s indispensable for molecular diagnostics, clinical research, and genetic studies.

Market Price

The Real-Time Quantitative PCR System YG1285 // YG1286 is competitively priced between $21,200 and $22,960 USD, depending on the configuration, accessories, and software options. This investment is ideal for laboratories seeking precision, efficiency, and reliability in customized multiplex detection and high-throughput capabilities.

Frequently Asked Questions

• What is the difference between the YG1285 and YG1286 models? The YG1285 has a 4-channel detection system, while the YG1286 includes an optional 5th channel for enhanced multiplex detection.
• What types of qPCR chemistries are compatible with this system? This system is compatible with FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, and Cy5.5 (optional for 5-channel models), supporting various probe-based and dye-based assays.
• Does the system require frequent calibration? No, its innovative spectrum separation technology minimizes the need for frequent calibration, ensuring stability and consistent performance over time.

Advantages and Disadvantages

Advantages:

• Fast and precise thermal cycling with a 6.0°C/s ramp rate
• High detection sensitivity capable of identifying single gene copies
• Multiplexing capability for up to 5 targets per reaction
• Advanced melting curve and gene expression analysis
• Excellent temperature uniformity and accuracy (±0.2°C)
• Wide compatibility with qPCR dyes and probes

Disadvantages:

• Higher initial investment compared to conventional PCR systems
• May require specialized training for advanced applications
• Limited to 96-well plate format, which may not suffice for ultra-high-throughput laboratories

Field Applications

The YG1285 // YG1286 Real-Time Quantitative PCR System is extensively used in:

• Clinical and Molecular Diagnostics – Pathogen detection, viral load quantification, and oncology biomarker analysis
• Pharmaceutical Research – Drug response studies, pharmacogenomics, and vaccine development
• Genetic and Forensic Analysis – Mutation detection, genotyping, and identity verification
• Food Safety and Agricultural Biotechnology – GMO detection, bacterial contamination analysis, and plant pathogen testing

Recommendations

For optimal performance and longevity of your PCR system, consider the following recommendations:

• Regularly clean optical components to maintain high-resolution fluorescence detection
• Use high-quality qPCR reagents to ensure consistent and reliable results
• Optimize assay conditions by validating reaction parameters before large-scale testing
• Perform firmware and software updates for enhanced system functionality

Technical Specifications