Description

The YG2139//YG2141 family represents excellence in real-time PCR systems, specifically designed to meet the experimental requirements of high-level laboratories, genetic research centers, and cutting-edge clinical diagnostic environments. With the capacity to process 96 samples per run, these units offer a comprehensive solution for multiplexed molecular detection.

Whether your laboratory requires maximum detection capacity with the 6 fluorescence channels of the YG2139 and YG2141 models, or an optimized 4-channel configuration with the YG2140 model, the series ensures absolute reliability. Its high-performance thermal control system and intuitive software allow specialists to perform complex analyses with unprecedented efficiency.

Design and Technology (or Functionality)

The YG2139//YG2141 systems integrate patented technologies that ensure consistent and precise results:

• Superior Scanning System: It utilizes a high-brightness, long-life, maintenance-free LED light source with detection via photodiodes (PDs). By performing the scan from the top, interference is minimized and the signal is maximized.
• Peltier Thermal Control: Reaches heating rates of up to 6.1 °C/s, which drastically reduces the total time of PCR protocols.
• Operational Flexibility: Dual control mode allows the equipment to be operated autonomously via its 10.4-inch touch screen or through a direct PC connection.
• Experiment Protection: The automatic resumption function after power failures ensures that valuable data and samples are not lost in the event of a supply failure.
• Advanced Multiplexing: Capability to detect up to 6 different targets in a single well (on E and T models), optimizing reagent use and time.

Frequently Asked Questions (FAQ)

1. What is the main difference between the model YG2139, YG2140, and YG2141? The difference lies primarily in the fluorescence channels. The YG2139 and YG2141 models feature 6 channels (ideal for complex clinical diagnostics and FRET), while the YG2140 offers 4 channels, covering the most common PCR applications.
2. Which consumables can I use? They are compatible with standard 0.2 ml 96-well plates, 8-tube strips, and individual tubes (transparent, frosted, or white).
3. How are result reports managed? The software includes reserved templates and allows for the creation of customized experiment reports that can be exported or printed directly following quantification or melting curve analysis.

Advantages and Disadvantages (Pros and Cons)

Advantages:

• Scanning Speed: Only 7 seconds for a full plate reading.
• Thermal Stability: Exceptional temperature uniformity of ±0.1 °C ensures reproducible results between wells.
• Autonomy: Large internal storage capacity for up to 1000 results without needing a PC connection.
• Versatility: Supports applications for absolute and relative quantification, SNP analysis, and melting curve analysis.

Disadvantages:

• Bench Space: With a width of 555 mm for some models, it requires considerable workspace compared to portable units.
• Energy Consumption: Requires 900 VA; therefore, the use of a stabilized UPS is highly recommended.

Field Use and Recommendations

These units are ideal for third-level hospitals, Centers for Disease Control (CDC), and pharmaceutical laboratories. They are the ultimate tool for detecting infectious pathogens, cancer biomarker research, and food safety testing.

Practical Tip: To ensure optical precision, always use high-quality optical consumables and ensure there is no condensation on the tube caps before starting the thermal cycle.

Technical Specifications