Description

The Real-Time Quantitative PCR System YG1285 // YG1286 is an advanced qPCR instrument that integrates innovative optical signal gathering, spectrum separation technology, and unique temperature control for high-sensitivity detection, precise temperature uniformity, and enhanced accuracy. Available in 4-channel and 5-channel configurations, this system is compatible with a wide range of qPCR dye-based and probe-based chemistries, allowing seamless multiplex PCR applications. The YG1285 and YG1286 models support absolute quantification, gene expression analysis, genotyping, HRM (High-Resolution Melting Curve Analysis), and MMCA (Multiple Mutation and Copy Number Analysis), making them essential for molecular diagnostics, clinical research, and genetic studies.

With a maximum ramping rate of 6.0°C/s, this high-speed qPCR system significantly reduces run times while maintaining exceptional temperature accuracy (±0.2°C). The SiPMT (Silicon Photomultiplier Tube) detector enables real-time fluorescence detection with extreme sensitivity, identifying as little as one gene copy with a 10-log dynamic range. Additionally, the system’s advanced melting curve analysis and multiplexing capabilities allow the detection of up to 5 targets in a single reaction, improving research efficiency and reducing reagent costs.

Market Price

The Real-Time Quantitative PCR System YG1285 // YG1286 is positioned in the market within a price range of $21.200 to $22.960 USD, depending on configuration, additional accessories, and software features. For laboratories requiring customized multiplex detection or high-throughput capabilities, this system represents an excellent investment in precision, efficiency, and reliability. To obtain a detailed quotation, request a personalized estimate through Kalstein Plus.

Frequently Asked Questions

• What is the difference between the YG1285 and YG1286 models?
  The YG1285 features a 4-channel detection system, while the YG1286 includes an optional 5th channel for expanded multiplex detection capabilities.
• What types of qPCR chemistries are compatible with this system?
  The YG1285 // YG1286 supports FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, and Cy5.5 (optional for 5-channel models), making it versatile for various probe-based and dye-based assays.
• Does the system require frequent calibration?
  No, the innovative spectrum separation technology minimizes the need for frequent calibration, ensuring long-term stability and consistent performance.

Advantages and Disadvantages

Advantages:

• Fast and precise thermal cycling with a 6.0°C/s ramp rate
• High detection sensitivity capable of identifying single gene copies
• Multiplexing capability for up to 5 targets per reaction
• Advanced melting curve and gene expression analysis
• Excellent temperature uniformity and accuracy (±0.2°C)
• Wide compatibility with qPCR dyes and probes

Disadvantages:

• Higher initial investment compared to conventional PCR systems
• May require specialized training for advanced applications
• Limited to 96-well plate format, which may not be sufficient for ultra-high-throughput laboratories

Field Applications

The YG1285 // YG1286 Real-Time Quantitative PCR System is widely used in:

• Clinical and Molecular Diagnostics – Pathogen detection, viral load quantification, and oncology biomarker analysis
• Pharmaceutical Research – Drug response studies, pharmacogenomics, and vaccine development
• Genetic and Forensic Analysis – Mutation detection, genotyping, and identity verification
• Food Safety and Agricultural Biotechnology – GMO detection, bacterial contamination analysis, and plant pathogen testing

Recommendations

For optimal performance and longevity, users should:

• Regularly clean optical components to maintain high-resolution fluorescence detection
• Use high-quality qPCR reagents to ensure consistent and reliable results
• Optimize assay conditions by validating reaction parameters before large-scale testing
• Perform firmware and software updates for enhanced system functionality

Technical Specifications