Product Description
The YG1285 and YG1286 Real-Time Quantitative PCR Systems stand at the forefront of modern laboratory technology, offering enhanced precision and efficiency for quantitative PCR applications. These systems are designed to meet the rigorous demands of scientists and researchers, delivering reliable and accurate data.
Both models boast a sample capacity that accommodates 8*0.1mL PCR strip*12, 96*0.1mL individual tubes, or a 0.1mL PCR Plate. The sample volume ranges between 10-50 μL, providing flexibility for various experimental requirements. Utilizing a Peltier-based heating and cooling method, these systems achieve a maximum ramping rate of 6.0° C/s across a temperature range of 4-100 °C. The temperature accuracy and uniformity are impeccable, maintaining ± 0.2°C and ±0.2°C@60°C ±0.3°C@95°C, respectively.
Furthermore, the optical detecting system of the YG1285 and YG1286 differentiates itself through time-resolved real-time scanning, with the YG1285 harnessing 4pcs, and the YG1286 utilizing 5pcs high-efficiency single color LEDs. Both models feature 4-5 detection channels and support a wide array of dyes including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, and Cy5/LIZ, with the option of Cy5.5 for 5-channel models. These systems provide sensitivity to detect as little as 1 copy gene and offer a robust dynamic range of 10 orders of magnitude.
| Model | YG1285 | YG1286 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
Considering the advanced capabilities and precision of the YG1285 and YG1286 PCR systems, their market price reflects their value. Typically, such technologically advanced PCR machines range from $15,000 to $30,000, depending on the customization and optional features selected. It’s advised to consult with authorized distributors for specific pricing tailored to your laboratory requirements.
Frequently Asked Questions
How do YG1285 and YG1286 differ in terms of LED excitation?
The YG1285 is equipped with 4 high-efficiency single-color LEDs, while the YG1286 boasts 5. This additional LED in YG1286 allows for a broader range of excitation, especially beneficial for complex analyses requiring more dye options.
Can the systems handle different types of samples?
Yes, both models are versatile and capable of handling various sample formats, including PCR strips, individual tubes, and PCR plates, accommodating a variety of experimental setups.
What kind of software support is available for these PCR systems?
Both systems are designed with user-friendly software interfaces that support a wide range of experimental protocols. Comprehensive technical support is available to ensure smooth operation and troubleshooting.
Advantages and Disadvantages
Advantages:
- High Precision: With a temperature accuracy and uniformity of ±0.2°C, these systems offer exceptional precision in temperature control, which is crucial for accurate PCR reactions.
- Versatile Optical System: The wide range of supported dyes and customizable detection channels allows for extensive experimental flexibility.
- Efficiency: The high-efficiency LEDs and fast ramping rates enhance overall throughput, making these systems ideal for high-demand labs.
Disadvantages:
- Cost: The advanced features come with a higher price point, which may be a constraint for smaller labs with limited budgets.
- Complexity: The extensive features and customization options may require additional training for users new to advanced PCR systems.
Use of the Product in the Field
The YG1285 and YG1286 are pivotal in various fields including biotechnology, genetics, and clinical diagnostics. In practical scenarios, these systems are used to amplify DNA, enabling researchers to conduct detailed analyses of genetic material. They are essential in diagnosing genetic diseases, conducting forensic investigations, and in agricultural research for crop improvement.
Researchers appreciate the systems’ ability to process a high volume of samples quickly, thanks to their efficient ramping rates and precise temperature control. This leads to expedited project timelines and faster results in high-pressure research environments.
Recommendations
For optimal performance and longevity of the YG1285 and YG1286 systems, regular maintenance and calibration checks are recommended. Operators should ensure proper handling and storage of samples and reagents. It is advisable to utilize the full range of supported dyes and LED channels for comprehensive analytical results.
Investing in periodic training for laboratory personnel can maximize the benefits of these sophisticated PCR systems, ensuring accurate data and efficient workflows. Additionally, consider utilizing compatible consumables and accessories that are recommended by the manufacturer to enhance the operational lifespan of the equipment.
Discover the innovation and quality that Yulgary has to offer. If you are looking for high-performance laboratory equipment, you are in the right place. At Yulgary, we manufacture products with standards of excellence that meet the most demanding needs. Browse our online store, designed to offer you a fast, easy shopping experience with competitive prices. Don’t miss the opportunity to enhance your laboratory with the best in scientific technology. Click here to explore our catalog.
