Product Description
The YG1279 Real-Time PCR System sets a new standard in molecular diagnostics with its advanced features and precision technology. Designed to deliver reliable and accurate results, this system accommodates various applications, including relative quantification, absolute quantification, melting curve analysis, and SNP analysis. Its throughput capacity is 48, making it ideal for laboratories looking for efficiency and effectiveness.
This system is equipped with two fluorescence channels, allowing for diverse dye compatibility. Channel 1 is compatible with FAM, SYBR Green I, SYTO 9, Eva Green, and LC Green, while Channel 2 supports HEX, VIC, TET, and JOE. The YG1279 boasts an impressive fluorescence scanning time of just 2 seconds, facilitated by a high-brightness, long-life, and maintenance-free LED light source, coupled with photodiodes (PDs) as detectors.
The thermal efficiency of the YG1279 is managed by a Peltier heating method, with a heating rate of ≥8.0 ℃/s and a cooling rate of ≥6.2 ℃/s. It further guarantees precision with a temperature accuracy of ≤0.1 ℃ and offers a gradient range from 1℃ to 40 ℃ across its 8-row gradient block. The system is compatible with various thermal procedures, such as thermal gradient PCR, long PCR, and Touch Down PCR, ensuring versatility for different testing protocols.
| Model | YG1279 |
| Throughput | 48 |
| Fluorescence Channels | 2 |
| Fluorescence Scanning Time | 2s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Optical System | |
| Light Source | High-brightness, long-life, and maintenance-free LED light source |
| Detector | Photodiodes (PDs) |
| Excitation Range | CH1: 470nm CH2:523nm CH3:570nm CH4:638nm |
| Detection Range | CH1:525nm CH2:564nm CH3:610nm CH4:685nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | ≥8.0 ℃/s |
| Cooling Rate | ≥6.2 ℃/s |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 8 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV ≤ 0.5% | |
| Software Functions | |
| Control Modes | Mode 1: 7-inch touchscreen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically resumes experiments after power restores, without PC software |
| Data Storage and Transmission | Upload and download via USB. Stores up to 1000 results |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60HZ; 600VA |
| Weight | 11 Kg (net) |
| Instrument Dimension | 260*400*260mm (W*L*H) |
| Suitable Consumables | 0.2mL clear non-skirt 48-well plates, 8-tube strips, single tubes, etc. |
Market Price
The Real-Time PCR System YG1279 is considered a premium product in the laboratory equipment market. Its price generally reflects the advanced technologies it offers, with market prices typically ranging from $10,000 to $15,000, depending on additional accessories or extended service plans included with purchase. It’s essential for potential buyers to consider factors such as warranty, customer service, and training when evaluating the cost-effectiveness of purchasing this equipment.
These PCR systems are a significant investment for any laboratory, intending to enhance their operational capabilities with robust and reliable PCR technology. Comparing offers from various suppliers can help laboratories find competitive prices and the best value for their investment.
Frequently Asked Questions
What is the throughput capacity of the YG1279 system?
The YG1279 system has a throughput capacity of 48 reactions, making it suitable for medium to high-volume laboratories seeking efficiency and precision in PCR experiments.
What kind of dyes can be used with this system?
This PCR system supports a wide range of fluorescent dyes. Channel 1 is compatible with FAM, SYBR Green I, SYTO 9, Eva Green, and LC Green, whereas Channel 2 accommodates HEX, VIC, TET, and JOE.
How is the instrument controlled?
The YG1279 offers dual control modes: a user-friendly 7-inch touchscreen and PC direct control, providing flexibility for varied user preferences and laboratory settings.
Advantages and Disadvantages
Advantages:
- The YG1279 features rapid fluorescence scanning time, enhancing laboratory productivity.
- Its optical system with long-life LED light source ensures durability and reduces maintenance costs.
- The Peltier heating method provides fast and precise temperature changes, saving valuable experiment time.
- Adjustable fluorescence dynamic range accommodates various sample requirements, from low to high concentrations.
- The automatic power failure protection feature provides peace of mind and operational continuity.
Disadvantages:
- The initial investment cost may be high, which could be a barrier for smaller labs or those with limited budgets.
- Advanced features may require training to ensure optimal use and understanding of the system capabilities.
Product Use in the Field
In practical scenarios, the YG1279 Real-Time PCR System is used widely across diverse molecular biology applications. Laboratories leverage its efficient throughput and high precision for a range of assays, from simple quantification studies to complex genotype assessments. The system’s flexibility with various dye chemistries allows researchers to customize assays according to specific experimental needs.
Additionally, the system’s robust and adaptable thermal cycling capabilities make it suitable for experiments that require precise thermal control, such as Touch Down PCR, ensuring consistent and reproducible results in various research settings.
Recommendations
To maximize the performance and longevity of the YG1279 Real-Time PCR System, it’s recommended to keep the system software up-to-date and follow regular maintenance schedules. Laboratories should ensure that personnel are adequately trained in both the operation and calibration of the equipment to maintain its precision engineering.
Moreover, having a backup power source could further safeguard against any unexpected power outages, ensuring that experiments are not disrupted. Regularly reviewing and understanding consumable compatibility will also help maintain smooth operational flow and prevent any technical issues related to sample loading and collection.
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